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电穿孔的英文

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"电穿孔"怎么读用"电穿孔"造句

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  • electroporation

例句与用法

  • Tagging cells with quantum dots by electroporation
    电穿孔法在量子点标记细胞中的应用
  • Analysis of the electroporation mechanism under the pulse electric field
    脉冲电场致细胞膜电穿孔的机理分析
  • In the direction of electromagnetic theory and the l of the mechanism of electroporation , the coaxial cavity resonator is be built by the means of cst microwave studio ?
    电穿孔理论和电磁理论为基础,采用cstmicrowavestudio ?建立电容加载同轴谐振腔的三维电磁模型,阐述基于此的灭菌方案。
  • The expression of lexa protein in the iptg - induced jm109 ( pza172 ) was checked by sds - page , and the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different concentrations of mmc
    应用电穿孔技术将携带有抗辐射菌lexa基因的重组质粒pza172转入大肠杆菌jm109 ,其启动子为lacz ,用iptg诱导, sds - page凝胶电泳检测lexa蛋白的表达。
  • The essentially universal biophysical phenomenon of " electroporation " occurs if an appropriate pulse field is applied . electroporation is believed to be the rapid creation of aqueous pathways through lipid - containing barriers in cells and tissue . the driving force is the physical interaction of electric fields with different dielectric constants
    电穿孔效应是指在适当高压脉冲电场作用下,细胞或组织间起相对隔离作用的“屏障”内快速形成液态通道的现象,是电场与具有不同介电常数而且易变形的物质相互作用的结果。
  • The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p . pastoris and transformed into p . pastoris by electroporation after linearization , 25 high - copied transformants were obtained by g418 screening . it was proved that the e2 genes were integrated stably into chromosome of p . pastoris by dot blot and dna sequencing
    猪瘟病毒e2基因的真核表达:分别将csfv两个代表株的e2基因克隆入毕赤酵母( p . pastoris )分泌型表达载体ppic9k中,酶切线型化后电穿孔导入p . pastotis进行整合,经g418筛选得到25个高拷贝转化子,经dna斑点试验和dna测序证明外源基因e2稳定地整合到p . pastoris染色体中。
  • According to the gene sequence and secondary structure of hcv ns5b , we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et . al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion , the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr
    然后根据dsrna设计原则,结合nssb基因的序列特征,借助生物信息学软件设计了针对nssb基因的sirnas ,并交由公司化学合成;电穿孔法转染上述稳定转染的细胞克隆,同时分别以非特异的sirnas转染组和空白转染组为对照, dapi染色后通过荧光显微镜和内标化rtpcr检测,初步证实了化学合成的sirnas可以特异阻断nssb基因的表达。
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