细胞培养液的英文

• cell culture fluid
• cell culture media

• "细胞"英文翻译    cell; sytes; bioplast; cella ...
• "培养液"英文翻译    culture solution; nutrient s ...
• "淋巴细胞培养液" 英文翻译 :    lcf; lymphocyte culture fluid
• "无血清细胞培养液" 英文翻译 :    serum-free cell culture medium
• "热灭活对照细胞培养液" 英文翻译 :    heat-inactivated control cell culture fluids
• "热灭活控制细胞培养液" 英文翻译 :    heat-inactivated control cell culture fluids; hicc
• "胸腺上皮细胞培养液" 英文翻译 :    tecf; thymic epithelial culture fluid
• "单细胞培养" 英文翻译 :    monocell culture; monocellular culture; single cell culture; single-cell culture; unicellular culture
• "肝细胞培养" 英文翻译 :    liver cell culture
• "活细胞培养" 英文翻译 :    biocytoculture
• "脓细胞培养" 英文翻译 :    biopyoculture
• "体细胞培养" 英文翻译 :    somatic cell culture
• "细胞培养" 英文翻译 :    biocytoculture; cell culture; culture of cells
• "细胞培养管" 英文翻译 :    cell culture tube
• "细胞培养罐" 英文翻译 :    cell factory; culture container
• "细胞培养基" 英文翻译 :    cell media
• "细胞培养瓶" 英文翻译 :    cell culture bottle; cell culture flask
• "细胞培养器" 英文翻译 :    cell cultivator; cell culture apparatus; cell factory; culture container
• "细胞培养物" 英文翻译 :    cc; cell cultures
• "细胞培养系" 英文翻译 :    lineage
• "血细胞培养" 英文翻译 :    hemoculture
• "体内细胞培养,活体细胞培养" 英文翻译 :    cell culture in vivo
• "玻片细胞培养" 英文翻译 :    slide cell culture
• "传代细胞培养" 英文翻译 :    continuous cell culture
• "次代细胞培养" 英文翻译 :    secondary cell culture

• After freezing and thrawing three times , the virus is concentrated by ultracentrifugation
  收集细胞培养液，并浓缩病毒。
• The number of adherent cells in special medium was more than that in common medium , but the morphologic difference between two groups was not significant at that time . the difference became significant after 24 hr of culture
  肝细胞培养液组贴壁的肝细胞数目明显多于普通培养液组，但此时两组培养液中的小鼠肝细胞在形态学上的差异并不显著。
• These cells were polygon and displayed morphologic characteristics of liver parenchymal cells at 24 hr of culture , such as a large round nucleus with a few nucleoli and many cytoplasmic granules , sometimes binucleate , hepatocyte was in direct contact with adjacent cells
  而肝细胞培养液组在低倍镜下观察，培养瓶的背景干狰，死细胞较少，贴壁细胞伸出多个伪足，呈多边形。相邻细胞连接成小片状。
• The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment . in brief , the intact blastocysts were plated on sto feeder layer treated with mitomycin , and were cultured in the media supplymented with brl condition medium
  联合evans和martin的方法，稍加改良来分离小鼠胚胎干细胞，把昆明白小鼠完整的囊胚直接种植在经丝裂霉素灭活的sto饲养层细胞上，在含有brl条件培养基的es细胞培养液中培养。
• Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml , es - d3 cells being used in our experiments formed normal clones , expressed akp and kept their normal karyotype over many passages . the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
  结果显示： eso3细胞在小鼠胚胎成纤维细胞上和或含白血病抑制因于亿f ）的es细胞培养液中形成典型的胚胎干细胞克隆，碱性磷酸酶染色结果为强阳性，具有正常二倍体核型以及具有在体内外分化为三个胚层来源的组织细胞的潜能，而且具有形成种系嵌合动物的能力。
• The primary results showed : using m199 as diluents containing 20 % bovine serum , it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example , from 0 to - 6 freezing speed is about - 0 . 05 a minute , from - 6 to - 40 , freezing speed is about - 0 . 5 a minute ) , studies on effect of various concentration of dmso demonstrate that about 12 . 5 % dmso gave the highest post - thaw percentage of viable cells . the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus . the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula . the cells preserved in liquid nitrogen at - 196 were thawed and cultivated , a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 % . the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
  研究初步表明:以细胞培养液m199 (含2既的小牛血清，常规量双抗)为冻存稀释液对泥鳅胚胎细胞冷冻保存宜采取先慢后快的方式(例如，从0一一6 ，冻存速度为一0 . 05 / min ，再以一0 . 5 / min的速度从一6一一40 ) ; dmso的保护效应浓度为12 . 506左右;小牛血清的浓度对泥鳅胚胎细胞的成活率影响不明显;囊胚晚期细胞抗冻性比中早期强;通过对不同批次的冻存细胞解冻培养，解冻后成活率为30 %以上细胞培养数天后均有少数细胞贴壁，但只发现两瓶培养细胞有明显增殖现象产生许多未分化的小细胞。
• Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus , two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196 . after 6 days cryopreservation , one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other , cryopreserved for 13 days , was 52
  以细胞培养液m199 (含20 %的小牛血清)为稀释液， dmso的浓度为11 % ;与泥鳅胚胎细胞冷冻保存方法一样，采取先慢后快的方式，冷冻保存两组草鱼囊胚晚期细胞于一1 %的液氮中。第一组冷冻保存6天后解冻，成活率为72 % ，第二组冷冻保存13天后解冻，成活率为520 / 0 。
• The eg cell culture media consisting of dmem medium supplemented with fbs , chicken serum , beta - mercaptoethanol , l - glutamine . hepes , chicken embryonic extract and cytokines etc . after 24 hours culture , the isolated pgcs were selectively attached on the gonadal stromall cells in the plates
  加入新鲜的eg细胞培养液(含dmem 、胎牛血清、鸡血清、 -巯基乙醇、 l -谷氨酰胺、 hepes 、鸡胚浸出液以及细胞因子等成分)培养24小时以后， pgcs开始部分贴附于共培养的生殖原基细胞上。
• Methods : hepatocytes obtained from mice with in situ portal vein collagenase perfusion were inoculated on two types of medium , which one was 10 % pcs rpmi 1640 medium ( common medium ) , the other was rpmi 1640 medium supplemented with 5 u g / ml tranferrin , 5 u g / ml insulin , 10nm nicotinamide , 5mm - me , 40 u g / ml hgf ( special medium )
  一种是在rpmi1640培养液中加入了511iml转铁蛋白、 5pg ml牛胰岛素、 10nm烟酚胺、 smm卜琉基乙醇， 4011g ml促肝细胞生长因子，简称为肝细胞培养液。