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蓝白斑筛选的英文

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"蓝白斑筛选"怎么读用"蓝白斑筛选"造句

英文翻译手机手机版

  • blue-white selection

例句与用法

  • The immunization effects of b95 can be disturbed by hi20 if the immunized ? interval less than 10 days with the normal dose
    将此片段与pbluescriptsk载体连接,转化大肠杆菌tgi ,经蓝白斑筛选,鉴定阳性克隆后测序。
  • 713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector . it was identified by restriction endonuclease digest analysis , pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene
    扩增产物连接到pgem - teasy载体上,转化入大肠杆菌jm109中进行蓝白斑筛选后,用酶切、 pcr鉴定和测序的方法鉴定出重组阳性质粒( pgem - hc和pgem - ha ) 。
  • The 1 . 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e . coli strains ( ff4169 ) . otsa gene is in charge of trehalose - 6 - phosphate synthesis in e . coli . in growth curve experiment , the transformants that carried the 1 . 488kb dna fragment grew well in minimum medium , which contains 0 . 5mol / l nacl , while control strains could n ' t endure it
    提取酿酒酵母的总dna ,以此为模板,采用pcr的方法从酿酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段,通过xba和sma双酶切,与同样经过xba和sma双酶切的puc118载体质粒连接,转入大肠杆菌dh5中,通过蓝白斑筛选重组子。
  • In order to construct the recombinant plasmid pcdna3 . 1 / ts87 , first , the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i , and kozak sequence . then the rebuilt ts87 fragment was cloned into t - vector and transformed into e . coli dh5 a
    通过重新设计引物在ts87两端加上用于构建重组质粒的酶切位点hind和bamh和用于真核表达的kozak序列。将改造后的基因片段克隆入t - vector ,转化大肠杆菌dh5 ,通过蓝白斑筛选获得阳性克隆后测序鉴定。
  • The e2 gene was amplified by rt - pcr , then examined the fragment by electrophoresis . after purification and insertion into pucm - t vecter , the recombinant plasmid pbne2pi and pbne2pii were obtained . then they were transfected e . coli jm109 and screened positive clones by blue or white plaques . the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis
    通过rt - pcr扩增e _ 2基因,电泳测定扩增片段的大小,经纯化后,连接于pucm - t载体,获得重组质粒pbne2p 、 pbne2p ,转化e ? colijm109 ,经蓝白斑筛选,挑取阳性克隆,提取质粒,直接电泳鉴定和酶切鉴定。
  • In this paper , a field strain of infectious bronchitis virus was isolated from proventriculus tissue , morphological observation by electron - microscope and the biological characterizations of the virus were studied , pairs of specific primers are designed and synthesized in correspondence with them , according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes , the cdna of si gene , s2 gene , m gene . n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
    在此基础上,根据国内外已发表的ibv基因序列,分别设计特异性引物,应用不同引物进行反转录合成cdna ,分片段对ibv的主要结构基因进行pcr扩增,并分别将各个目的片段克隆到puc19载体上,在大肠杆菌dh5中实现目的基因的分子克隆,经蓝白斑筛选、限制性内切酶分析、 pcr鉴定,筛选出重组阳性质粒,并对各个目的基因片段进行序列测定,从而获得ibv主要结构基因全序列。
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