蓝白斑筛选的英文
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"蓝白斑筛选"怎么读用"蓝白斑筛选"造句
英文翻译手机版
- blue-white selection
- "白斑"英文翻译 hickie; leukasmus; vitiligo; ...
- "筛选"英文翻译 dressing by screening; scree ...
- "蓝白" 英文翻译 : blue white; lanshi
- "白斑" 英文翻译 : hickie; leukasmus; vitiligo; floccosoids (如铁杉)◇白斑病 leucoplakia; leucoderma; leucodermia; melanodermia; piebald skin; vitiligo
- "筛选" 英文翻译 : dressing by screening; screen; preparation by screening; preparation; choose by means of a sift; to sift 经过反复筛选, 选出近一百篇可能入选的小说。 the repeated siftings have brought the number of possible stories to the neighbourhood of one hundred.; 筛选程序 screening sequence; 筛选等级 screening class; 筛选规格 screening specification; 筛选机 screening machine; sieving machine; 筛选进口商品 screening of import commodities; 筛选累加 filtered accumulation; 筛选器 screening washer; 筛选砂 riddled sand; 筛选试验 screening test; 筛选条件 screening conditions; 筛选效率 screening efficiency; 筛选装置 screening plant
- "蓝白热" 英文翻译 : blue-white heat
- "蓝白烟" 英文翻译 : white and blue smoke
- "蓝白钻" 英文翻译 : blue white; bluewhite
- "天蓝白" 英文翻译 : azure
- "泛蓝白化" 英文翻译 : bloom
- "甘蓝白锈病" 英文翻译 : white rust of cabbage
- "蓝白矮星" 英文翻译 : white-blue dwarf
- "蓝白方格布" 英文翻译 : cranky checks
- "蓝白管土" 英文翻译 : camstone
- "蓝白海豚" 英文翻译 : striped dolphin
- "蓝白花布" 英文翻译 : indigo print
- "蓝白军团" 英文翻译 : albiceleste
- "蓝白玛瑙" 英文翻译 : nicolo
- "蓝白南燕" 英文翻译 : blue-and-white swallow
- "蓝白色钻石" 英文翻译 : bluewhite diamond
- "蓝白特投影" 英文翻译 : projection; lambert
- "蓝白足球会" 英文翻译 : gd lam pak
- "蓝白钻石" 英文翻译 : blue white
- "蓝白翡翠" 英文翻译 : glittering kingfisher
- "美国蓝白子" 英文翻译 : american blue
例句与用法
- The immunization effects of b95 can be disturbed by hi20 if the immunized ? interval less than 10 days with the normal dose
将此片段与pbluescriptsk载体连接,转化大肠杆菌tgi ,经蓝白斑筛选,鉴定阳性克隆后测序。 - 713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector . it was identified by restriction endonuclease digest analysis , pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene
扩增产物连接到pgem - teasy载体上,转化入大肠杆菌jm109中进行蓝白斑筛选后,用酶切、 pcr鉴定和测序的方法鉴定出重组阳性质粒( pgem - hc和pgem - ha ) 。 - The 1 . 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e . coli strains ( ff4169 ) . otsa gene is in charge of trehalose - 6 - phosphate synthesis in e . coli . in growth curve experiment , the transformants that carried the 1 . 488kb dna fragment grew well in minimum medium , which contains 0 . 5mol / l nacl , while control strains could n ' t endure it
提取酿酒酵母的总dna ,以此为模板,采用pcr的方法从酿酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段,通过xba和sma双酶切,与同样经过xba和sma双酶切的puc118载体质粒连接,转入大肠杆菌dh5中,通过蓝白斑筛选重组子。 - In order to construct the recombinant plasmid pcdna3 . 1 / ts87 , first , the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i , and kozak sequence . then the rebuilt ts87 fragment was cloned into t - vector and transformed into e . coli dh5 a
通过重新设计引物在ts87两端加上用于构建重组质粒的酶切位点hind和bamh和用于真核表达的kozak序列。将改造后的基因片段克隆入t - vector ,转化大肠杆菌dh5 ,通过蓝白斑筛选获得阳性克隆后测序鉴定。 - The e2 gene was amplified by rt - pcr , then examined the fragment by electrophoresis . after purification and insertion into pucm - t vecter , the recombinant plasmid pbne2pi and pbne2pii were obtained . then they were transfected e . coli jm109 and screened positive clones by blue or white plaques . the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis
通过rt - pcr扩增e _ 2基因,电泳测定扩增片段的大小,经纯化后,连接于pucm - t载体,获得重组质粒pbne2p 、 pbne2p ,转化e ? colijm109 ,经蓝白斑筛选,挑取阳性克隆,提取质粒,直接电泳鉴定和酶切鉴定。 - In this paper , a field strain of infectious bronchitis virus was isolated from proventriculus tissue , morphological observation by electron - microscope and the biological characterizations of the virus were studied , pairs of specific primers are designed and synthesized in correspondence with them , according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes , the cdna of si gene , s2 gene , m gene . n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基础上,根据国内外已发表的ibv基因序列,分别设计特异性引物,应用不同引物进行反转录合成cdna ,分片段对ibv的主要结构基因进行pcr扩增,并分别将各个目的片段克隆到puc19载体上,在大肠杆菌dh5中实现目的基因的分子克隆,经蓝白斑筛选、限制性内切酶分析、 pcr鉴定,筛选出重组阳性质粒,并对各个目的基因片段进行序列测定,从而获得ibv主要结构基因全序列。
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