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通用引物的英文

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"通用引物"怎么读用"通用引物"造句

英文翻译手机手机版

  • consensus primer
  • universal primer

例句与用法

  • The positive clone is selected by colony pcr and dna sequence is analyzed and determined by the m13 primer
    Coltjm109感受态细胞,菌落pcr筛选鉴定阳性克隆,用m13通用引物进行dna序列测定。
  • Materials and methods in this study , we perform 18 cases of chordoma tissue and its normal tissue around them as materials . genomyx fluoro dd kits are from beckman compa - ny , usa
    选用通用引物竹和m13对纯化后cdnai和cdnaz进行测序,测序结果大小分别为1045bp和870bp ,与纯化结果一致。
  • It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent , genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study , genetic diversity in a . polytricha was higher than that in a . auricula : 4 in this study , a . fuscosuccinea had a higher homology to a . auricula than to a . polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a . auricular and a . fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a . auricula as a probe which hybridized with a . auricula and a . fuscosuccinea except a . polytricha , further confirming the veracity of the results from eric - pcr ; 7 in this study , isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
    本研究中,木耳属2个种的2个菌株在its区域表现出较高的保守性, 4种限制型内切酶的酶切图谱没有显示出多态性;增加内切酶种类及供试菌株数量,有可能获得具有多态性的限制性内切酶酶切图谱; 9本实验中, its区域的真菌特异性引物与真核生物通用引物对于扩增效果无较大差异,扩增片段长度均为650bp左右; 10根据形态学实验、 eric - pcr实验以及southern杂交实验的结果分析,紫木木耳属种质资源的遗传鉴定和遗传多样性评价耳极有可能是毛木耳种的一个变种; n .本研究中所用的gutc法是一种适用于木耳属菌株基因组洲a快速提取的方法; 12 .传统的形态学分类法和现代的分子生物学分类法,两者的关系是相辅相成,互为验证
  • Based upon the comparison of cyto b gene sequences in 15 deer species downloaded from genbank , a universal primer set l15774 / hsf21 was used as positive control of the template quality , at the meantime , two species specific primer sets df / dr and cf / cr were desgined to identify red deer ( cervus elaphus ) , sika deer ( cervus nippori ) and roe deer ( capreolus capreolus ) from other species . a musk deer ( moschus ) specific primer set wf / mr was designed , siberian musk deer ( afoschus moschiferns ) and forest musk desr ( moschus berezovskii ) could be discriminated when restriction endonuclease rsa i was used to cut the pcr products
    经过对来自genbank中的15种鹿类动物的cytob基因序列的比较,用通用引物l15774和hsf21作为模板的质量控制,设计了特异性引物df dr和cf cr来鉴定马鹿、梅花鹿、狍;设计了麝类特异性引物mf mr ,用限制性内切酶rsa酶切扩增产物来区分原麝和林麝。
  • The hemaglutinin ( ha ) of aiv plays the key role in determing the pathogenicity , cell receptor binding property and host range of the virus . the homology of the ha sequences reported and registered in genbank of different strains of h _ ( 5 ) . h _ ( 9 ) subtype was respectively analyzed and compared with each other . the conservative domin of ha seqence of h _ ( 5 ) , h _ ( 9 ) subtype was selected for pcr amplification . two sets of specific primers were designed
    本研究根据genbank中查出的禽流感病毒h _ 5亚型,禽流感病毒h _ 9亚型的ha片段基因组序列,利用dnasis软件分别对aiv的h _ 5 、 h _ 9亚型ha基因区域进行同源性比较,设计筛选出两对联合pcr反应的特异性引物,其中一对是h _ 9亚型通用引物,扩增出ha片段695bp ,另一对引物为h _ 5亚型的通用引物,扩增出的ha区域部分目的片段约为448bp 。
  • Highly conserved sequences in the coding regions of the phac genes have been found by gene alignment ofphac genes cloned from 5 known pseudomonas spp . , and these highly conserved sequences can be used as primers for the pcr screening of phacl / phac2 - containing microorganisms . touch - down pcr has been adopted for the cloning of type ii pha synthase genes
    根据这些保守区的序列设计出两条用于pcr扩增的通用引物,以细菌的基因组dna为模板,采用特殊的pcr实验方案降落pcr ( touch - downpcr )对型pha合酶基因进行了成功地克隆。
  • In this paper , total dna from tannage , tail skin , scales and the skin treated with salt were successfully extracted with an improved method for dna extraction . to verify the results , four pairs of primers , which were universal primers for 12s rrna gene , diagnostic primers of chinese alligator meat , microsatellite primers and rapd primer , were used to do pcr amplifications . some amplified fragments were sequenced , either
    本文研究出一种改进的dna提取方法,成功地从扬子鳄鞣制皮革中提取了总dna ,同时对不同的组织标本如鳞片、盐腌生皮及尾尖皮等进行了dna提取:并利用12srrna基因扩增的通用引物、扬子鳄鉴定性引物、微卫星引物及rapd引物进行pcr扩增,且对部分pcr扩增结果进行测序,以检验提取效果。
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