Effect of total coumarins of fructus cnidii on osteoblast in neonatal rat calvaria culture 蛇床子总香豆素对新生大鼠成骨细胞的作用
The insulin-like growth factor-2 ( igf-2 ) and igf conjugated protein-6 ( igfbp-6 ) mrna level in rat calvaria bone tissue and mc-3t3-el cells were detected by northern blotting analyses and reverse transcription polymerase chain reaction . the estrogen responsive element ( ere ) in igfbp-6 gene promoter was identified and involved in tcdd-reduced regulation of the gene expression by electromobility shift assays ( emsa ) 正常胎鼠头盖骨组织igf一2mrna呈高水平表达状态,而igfbp一6mrna的水平较低;ccf胎鼠头盖骨骨组织内igf一2mrna的表达较正常胎鼠降低,igfbp一6mrna的表达则明显升高;atra和e:联合应用时,atra可以抑制雌激素对细胞内igf一2和igfbp一6的这种调节作用。
The protein nucleic acid complexes were identified by autoradiography for 48 h . the psg5heo and igfbp-6 promoter pcat vectors were transfected into cos-7 cells with iipofectamin2000; these cos-7 cells where treated with 1 jxm e2, with lonm tcdd and with both lonm tcdd and 1 jxm e2, individually; the cat protein was examined by cat-els a . results : the congenital skeletal defects in fetal rats were induced with 10-15ug / kg tcdd or with 100-140 mg / kg ra with dosage-dependence effect . the igfbp-6 mrna in rat calvaria tissue and mc-3t3-el cells were increased with tcdd and ( or ) ra, whereas the cell proliferation and igf-2 mrna in mc-3t3-el cells was decreased in the status of e2 结果:tcdd在大鼠胚胎发育时期具有骨骼致畸作用,骨骼发育畸形包括:内翻足、脊柱裂、颅面骨发育缺陷、无尾畸形等;随着tcdd浓度的升高(10一15林扩kg),致畸率明显上升(14.7%一24.6%);tcdd和ra联合用药可以增强对大鼠骨骼发育的致畸效应;e:对成骨细胞内igf一2基因有正性调节作用,对igfbp一6基因有负性调节作用,tcdd可以抑制e:对细胞内xgf一2和igfbp一6的调节作用。