Fluoropolymer - based coatings are usually based on a dispersion of finely - divided resin particles in dispersants and diluents 含氟树脂涂料是在分散剂和稀释液中很好的分散树脂粒子所形成的涂料。
If an mpn is performed distribute 10 ml each of the first dilution in three sterile tubes and prepare additional dilutions in bpw as needed 如分析mpn ,分别取三个10毫升- 1倍稀释液于无菌试管,根据要求准备另外的稀释液于bpw 。
Milk and milk products - general guidance for the preparation of test samples , initial suspensions and decimal dilutions for microbiological examination 乳和乳制品.用于微生物检验的试验样品初始悬浮液和十倍制稀释液的制备的通用指南
Microbiology of food and animal feeding stuffs - preparation of test samples , initial suspension and decimal dilutions for microbiological examination - specific rules for the preparation of meat and meat products 食品和动物饲料的微生物学.用于微生物检验的试验样品的初始悬浮液和十倍稀释液的制备.肉和肉制品制备的特殊规则
We also studied the following from four aspects including the model of breeding , the time of enjecting oxytocic hormone , the effect of diluent in fertilization and the model of hatching 我们从亲本饲养模式、判断催产时机、受精稀释液对精子的影响、受精方法和孵化模式等4个方面进行了研究和探讨,以期完善大鲵的人工繁殖技术。
Microbiology of food and animal feeding stuffs - preparation of test samples , initial suspension and decimal dilutions for microbiological examination - general rules for the preparation of the initial suspension and decimal dilutions 食品和动物饲料制品微生物学.试样制备及用于微生物检验的初始悬浮液和十倍制稀释液.初始悬浮液和十倍制稀释液制备的一般规则
Microbiology of food and animal feeding stuffs . preparation of test samples , initial suspension and decimal dilutions for microbiological examination . part 1 : general rules for the preparation of the initial suspension and decimal dilutions 食品和动物饲料的微生物学.用于微生物检验的初始悬浮和十倍稀释试样的制备.第1部分:初始悬浮和十倍稀释液制备的一般规则
With the analysis of the document , it was clear that the variation of weight was one of the objective standards of determing the time of hasten parturition . in practice the variations of weight of every parent should be surveyed as a scientific basis of hasten parturition 3 .低浓度的hoif氏原液和井水(浓度待测)能明显促进精子运动;较高浓度的ringer氏原液妨碍精子运动。 4 .用干湿法授精,添加精子稀释液。
The primary results showed : using m199 as diluents containing 20 % bovine serum , it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example , from 0 to - 6 freezing speed is about - 0 . 05 a minute , from - 6 to - 40 , freezing speed is about - 0 . 5 a minute ) , studies on effect of various concentration of dmso demonstrate that about 12 . 5 % dmso gave the highest post - thaw percentage of viable cells . the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus . the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula . the cells preserved in liquid nitrogen at - 196 were thawed and cultivated , a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 % . the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells 研究初步表明:以细胞培养液m199 (含2既的小牛血清,常规量双抗)为冻存稀释液对泥鳅胚胎细胞冷冻保存宜采取先慢后快的方式(例如,从0一一6 ,冻存速度为一0 . 05 / min ,再以一0 . 5 / min的速度从一6一一40 ) ; dmso的保护效应浓度为12 . 506左右;小牛血清的浓度对泥鳅胚胎细胞的成活率影响不明显;囊胚晚期细胞抗冻性比中早期强;通过对不同批次的冻存细胞解冻培养,解冻后成活率为30 %以上细胞培养数天后均有少数细胞贴壁,但只发现两瓶培养细胞有明显增殖现象产生许多未分化的小细胞。
Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus , two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196 . after 6 days cryopreservation , one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other , cryopreserved for 13 days , was 52 以细胞培养液m199 (含20 %的小牛血清)为稀释液, dmso的浓度为11 % ;与泥鳅胚胎细胞冷冻保存方法一样,采取先慢后快的方式,冷冻保存两组草鱼囊胚晚期细胞于一1 %的液氮中。第一组冷冻保存6天后解冻,成活率为72 % ,第二组冷冻保存13天后解冻,成活率为520 / 0 。