cpti中文是什么意思

• 三大协会及美国钢管进口委员会

• cpts    中国学龄前儿童气质量表
• cptc central processor test console    中央处理机测试控制台
• cptt    职业培训师
• cpta    中国包装技术协会
• cpt定理    cpt theorem
• cpt-1    酰转移酶
• cpu    CPU ＝ Central Processing Unit 【自动化】中央处理机。
• cpt(carriage paid to)    运费付至
• cpu architecture    中央处理机体系结构
• cpt william lennicks    兰尼克斯上尉

• 4 . the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi , and collected the digested cpti fragment and the pgem - 7z , then ligated by t4 dna ligase and formed the pgem - cp
  中间载体及表达载体的构建将puc - cp质粒和pgem ? 7z质粒，用kpni和bamhi酶切，分别回收cpti片断和酶切后的载体片段，用t _ 4连接酶连接构建成中间载体pgem - cp 。
• For there is no anti - cpti antibody to use for the assay comparing with the antibody which was obtained by nucleic acid immunization , the work that the cpt / qene was expressioned in e . co / / ar d purified the protein for antibody preparation was carried out at the same time
  鉴于没有现成的抗cpti抗体与我们获得的核酸免疫抗体进行比较试验，本研究同时进行了cpti基因的细菌表达与蛋白纯化用于抗体制备。
• 2 . cloning of the pcr products the pcr products were purified by agarose gel electrophoresis and was ligated with pucm - t vector . by the method of pcr and enzyme digest analysis . the result shows that the plasmid containing cpti gene was transferred into e . coli dhso
  Pcr产物的克隆采用a / t克隆法，将pcr产物经琼脂糖凝胶电泳纯化回收后用t4连接酶与pucm - t载体连接，构建成克隆载体puc - cp ，转化大肠杆菌dh _ 5 。
• By immune the animal with the protein , the antibody which is anti - gst - cpti fusion protein was prepared . lt was testified that the antibody can special immunological recognition the protein gst - cpti , gstand cpti by indirect elisa . the coefficient of correlation is significant and the potency is more than 1 : 8000
  对其进行细菌表达后，利用glutathionesepharose4b亲和柱纯化获得gst - cpti融合蛋白，免疫动物后制备了相应的抗gst - cpti融合蛋白抗体。
• The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene . the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it
  豇豆胰蛋白酶抑制剂( cpti )基因是目前在植物抗虫基因工程中应用仅次于bt基因的广谱性抗虫基因。鉴于它抗害虫的广谱性和靶标昆虫不易对其产生耐受性的优点，转cpti基因植物得到了迅速的发展。
• A eukaryotic expression vector pcdna3 . 1 - cptl was constructed by insert cp77 gene into the vector pcdna3 . 1 which is used in nucleic acid immunization . the vector was immuned the balb / c mice by the method intramuscular injection after extracted and purified in great deal . immu - nological reaction was induced by the expression of cptl after the vector entered into the mice body
  本研究通过限制酶将cpti基因片段从载体pbluel3上切下，插入真核表达载体pcdna3 . 1 ，构建了用于核酸免疫的真核表达载体pcdna3 . 1 - cpti ；质粒大量提取和纯化后，通过肌肉注射的方法免疫balb c小鼠，基因表达产物刺激小鼠机体产生免疫反应，从而获得了抗cpti蛋白的抗体。
• With the base of related research on nucleic acid immunization , the technology was used to develop th e research and application of cpti transgenic plant . a new antibody preparation method by nucleic acid immunization to assay the expression of the transgenic gene was explored and compared with the traditional one which immune animal by protein
  在国内外大量核酸免疫的研究基础上，本研究首次将核酸免疫技术应用于转基因植物检测研究中，探讨一种核酸免疫法制备抗cpti抗体来检测基因表达的方法，并与传统的蛋白免疫方式制备抗体进行比较。
• Now the biological and molecular assays are the major methods to assay cpti transgenic plant . there is no better method on assaying the expression of the transgene . the reason would be that the outcome of expression by cpti is a little peptide and its molecular weight is very small . its immu - nogenicity would be insufficiency so that it can not induce strong immu - nological reaction and can not produce special antibody of high potency
  目前对转cpti基因植物的检测主要集中在生物测定和分子检测，尚无较好的基因表达产物的检测方法，其主要原因在于cpti基因的表达产物是小分子多肽，分子量小，免疫原性不够，直接免疫不足以产生较强的免疫反应，不能产生效价高的特异性抗体。
• So , a new method to preparation anti - cpti antibody by nucleic acid immunization in assay the expression of c ? 77transgenic rice can be established . for there are some problems that the potency of the antibody prepared by nucleic acid immunization is not high enough and there is highly background in assay the expression of cpti transgenic rice , more and further researchs should be carry out
  本研究初步建立一种核酸免疫法制备抗cpti抗体，并用于转今万基因水稻基因表达产物检测的方法。鉴于目前制备的核酸免疫抗体效价仍不高，以及转基因水稻中存在本底等问题，需要进一步深入研究。
• By the same method , the expression vector pbi121 - cp was constructed from pgem - cp and pbi121 with xbal and saci digestion . after that , the pbi121 - cp was transferred into agrobacterium lba4404 strain by freeze - thaw method . the pcr amplification indicated the lba4404 strain containning cpti gene . the lba4404 strain was used in genie transformation of mustard
  经酶切和pcr扩增验证后，以xbai和saci双酶切pgem ? cp和pbi121 ，将切下的cpti片段和pbi121载体片段连接构建成表达载体pbi121 - cp ，用冻融法导入农杆菌lba4404 ，提取质粒，经pcr扩增检测，用于芥菜的遗传转化。