nested pcr中文是什么意思

• 巢式pcr
• 嵌套式聚合酶链式反应

• nest    n. 1.巢；窝；窟,穴。 2.安息处,休息处；住处,家 ...
• pcr    PCR ＝polymerase chain reacti ...
• rt-nested pcr    逆转录-套式聚合酶链反应
• nested    巢状的; 内装的; 嵌套的; 相互套入的
• as-pcr    等位基因特异性多聚酶链反应
• pcr    PCR ＝polymerase chain reaction 【生物化学】聚合酶联锁反应〔此项技术可用以复制犯罪现场发现的DNA小样，从而有助于破案〕。
• nested barges    套装驳
• nested binding    嵌套结合
• nested block    嵌套分程序; 嵌套块; 嵌套循环
• nested blocks    嵌套块
• nested boats    套放救生艇
• nested caldera    巢状破火山口
• nested cell    嵌套单元
• nested class    巢状类别嵌套类; 有关
• nested classification    抱窝型分类
• nested code    嵌套码
• nested conditions    嵌套条件
• nested construct    嵌套构造
• nested crater    巢状火山口
• nested craters    巢状火山口
• nested data    数据套
• nested dc    多套
• nested decision    嵌套判定
• nested deletion    二十四碳单烯酸; 嵌套缺失
• nested deposit    巢状矿床

• Part 1 : identification of a novel gene , tsarg2 , and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell , and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis . to rapidly attain human novel gene full - length cdna sequence , the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe , which was significantly changed in cryptorchidism and represents a novel gene . furthermore , a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
  本研究分为三个部分，其主要实验方法及实验结果如下：第一章tsarg2基因的克隆与序列分析从已获得的在隐睾和正常睾丸对照中表达量有明显差异的est片段( be644542 )入手，设计了基因特异性引物和载体特异性引物进行巢式pcr扩增，结合人类基因组草图搜索法，从睾丸cdna文库中快速分离出人类睾丸凋亡相关基因的5末端而获得全长cdna ， genbank登录号为ay040204 ，同时应用生物信息学的方法克隆了该基因在小鼠中的同源基因， genbank登录号为af395083 。