Absorption , fluorescence emission and excitation spectra of five pigment - protein complexes were determined . photosynthetic electron transfer was measured from the dcip photoreduction . p700 concentration was assayed from the ferricyanide - oxdised minus ascorbate - reduced difference spectrum 测定裙带菜各色素蛋白复合物的吸收光谱、荧光发射光谱和荧光激发光谱,并进行了dcip的光还原测定和化学法的氧化还原差示光谱测定。
Now , in a study that took more than five years to complete , rockefeller university researchers , in collaboration with a team of bacteriologists at the university of wisconsin , madison , have become the first to solve the structure of a protein complex that protects these cells from singlet oxygen 现在,洛克菲勒大学的研究人员与威斯康星大学的细菌学家一同协作,历经5年多时间的研究,首次获得了一种能够保护细胞免受单态氧损伤的蛋白复合体的晶体结构。
Quantitatively describing the principle of protein - protein interactions and recognition in molecular level is the key point for understanding the relationship between structure and function of protein complexes and designing protein complexes , hi these interactions among proteins , electrostatic and hydrophobic interactions are the two most important ones , which let protein molecules bond systematically 在分子水平上定量地阐明蛋白质分子之间的亲合力和相互识别的机制,是正确地认识蛋白质聚集体结构和功能之间的关系并合理地设计出有用的蛋白质分子聚集体的关键。蛋白质分子间相互作用,其中最主要的是静电和疏水相互作用,使蛋白质分子能有序地结合在一起。
The chemical components of silkworm pupa crust were analyzed , and its microstructure was characterized by using scanning electron microscope . the existing realtion of among chitin 、 protein and inorganic salt in silkworm pupa crust has been observed . the results show that the major protion of silkworm pupa chitin is in pupa crust , and it accounts for about one forth of crust weight , the out surface of pupa crust is regular polygon net vein characteristics . chitin takes honeycomb shape in chitin - protein complex and conjugated with protein . the inner space of chitin - protein complex net was filled with inorganic salts . thus the theory basis was provided for working out the process route of isolation pupa chitin 对桑蚕蛹皮的成分、结构进行了化学及扫描电镜分析,确定其含有的主要成分及含甲壳素的数量,并对其中的甲壳素、蛋白质和无机盐三者之间的存在方式进行了观察.研究结果表明,蛹体中的甲壳素与灰分主要含在蛹皮中,甲壳素占整个蛹体成分的2 . 71 % ,占蛹皮重量的25 . 5 % ,蛹皮外表面呈规整的多边形网状结构,蛹皮中蛋白质与蜂窝状的甲壳素相结合,呈层状分布,颗粒状的无机盐填充在甲壳素/蛋白质复合物构成的蜂窝状的空隙中.这为制定提取蛹甲壳素的工艺路线提供了理论依据
To solve this problem , we used the formal charge model to study electrostatic interactions of protein complexes . and a fast and effective model for predicting the salt and ph dependent properties of protein complexes was presented here and applied to the analysis and prediction of the stability of protein structures 针对这个问题,我们采用简单形式电荷模型来研究蛋白质二聚体静电相互作用,建立了一种快速、有效地预测蛋白质稳定性随溶液ph值和盐离子浓度变化的方法,并将其应用于蛋白质分子结构稳定性的分析和预测。
Taking two protein complexes , we performed md simulations on their candidate structures . according to the variations of the mean square deviation ( msd ) of the structures in md trajectories relative to the initial structures , the false structures can be excluded 以两种蛋白质复合物为例,对其候选结构进行md模拟,根据md轨迹中构象相对于初始北京工业大学工学硕士学位论文摘要一构象的平方平均偏差( msd )随时间的变化来辅助打分函数排除错误构象,得到了较好的结果。
Our goal is to establish a simple and effective empirical approach to calculate the conformational entropy and the binding free energy by analysis of j : protein interface . three variables of the binding interface information of 20 protein complexes , including the binding accessible number ( nb ) , hydrophilic pair ( n ^ , ) and apolar solvent - accessible surface areas ( & asaapol ) are analyzed 本论文在此分析了20组蛋白质二聚体分子的结合界面三个有效的参数信息,即界面残基侧链可接触数( n _ b ) 、亲水对数( n _ ( pair ) )和非极性溶剂可接近表面积的变化( asa _ ( apol ) ) 。
The main concept of this work includes the following aspects : ( 1 ) based on the soft - docking algorithm which is improved upon wodak and janin ' s protein - protein rigid docking algorithm , we took seventeen protein - protein complexes for examples to study the method how to select the near - native structure from docking conformations 本论文的主要研究内容如下: 1采用本组在wodak和janin的刚性对接算法基础上改进的半柔性对接算法,以17种蛋白质-蛋白质复合物体系为例,我们研究了如何正确有效地从对接采样中挑选出近天然构象的方法。