The random amplified polymorphic dna ( rapd ) and inter - simple sequence repeat ( issr ) analysis were used to estimate population genetics of fenneropenaeus chinensis . we analyzed the genetic diversity and genetic structure of the populations , and also studied the genetic divergency among populations and geographic populations . the main results shown as follows : 1 本文以中国对虾( fenneropenaeuschinensis )野生群体为研究对象,采用rapd和issr分子标记技术,进行了群体遗传学的研究,对中国对虾野生群体的遗传多样性及群体遗传结构进行了分析,研究了中国对虾各野生群体之间的遗传分化情况。
Cluster analysis by upgma methods indicated that these five stocks might be divided into three clusters . results of rapd a nalysis suggested that extensive genetic diversity was detected in this species and the genetic divergence among stocks was relatively high ; upgma phylogenetic tree showed there existed three geographic populations of p . polyactis in the yellow sea and the east china sea , which supported the previous conclusion by morphological and ecological methods . part two : the genetic diversity between trichiurus lepturus and eupleurogrammus muticus random amplified polymorphic dna ( rapd ) technique was applied to 12 individuals from each species of the hairtail fishes trichiurus lepturus and eupleurogrammus muticus collected from the yellow sea 50一2 . 44 ) ,群体内和群体间的遗传变异比例分别为69 %和31 % ;群体间的平均遗传相似度和遗传距离分别为0 . 9139和o . q861 ;用非加权配对算数平均法( unweightedpair - groupmethodofari山m七tiome即s , upgma )聚类分析的结果表明,所分析的5个群体可被分为3个地理群系,从分子水平上支持了过去有关学者把黄海和东海的小黄鱼划分为北中南3个地理群系的观点。
Rapd ( random amplified polymorphic dna ) technique was used to study the genetic structure and genetic differentiation of the natural population and the hatchery stock of chlamys farreri . amplifications with 20 10 - base primers under predetermined optimal reaction yielded 153 reproducible amplified fragments ranging from 200 to 3000bp . the mean proportions of polymorphic amplified fragments of natural population and the hatchery stock were 73 . 2 % and 70 . 6 % respectively , and the mean hetreozygosities of them were 0 . 27 and 0 . 26 respectively 在对20个野生栉孔扇贝和20个养殖栉孔扇贝的基因组dna的检测中, 20个随机引物共扩增出153条清晰可分辨的dna片段,片段大小在200 3000bp之间,其中野生种群和养殖群体多态性片段分别为112和108条,多态位点比例分别为73 . 2 %和70 . 6 % ,杂合度分别为0 . 27和0 . 26 。
Random amplified polymorphic dna ( rapd ) reactions were performed on the total 27 samples including 23 accessions mentioned above ( no . 1 - 21 , 26 ~ 27 ) , 1 accession of lchinense var . semper - rubrum ( no . 22 , another variety of lchinense } , 1 accession from a branch with green leaf white flower on a plant which has the similar morphological trait with no . l ( no . 25 ) , and 2 accessions of lchinense ( no . 23 ~ 24 ) 对27个材料包括: 23个红?木变异类型, 1个长红?木( loropetalumchinensevar . semper - rubrum )材料? ? ?木的另一变种, 1个采自与1号形态类似的红?木植株上的绿叶白花的变异枝条,以及2个原种?木材料做了随机多态扩增dna ( rapd )分析。
Rapd ( random amplified polymorphic dna ) , which bases on the polymerase chain reaction ( pcr ) , is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms . the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna , and a small number of fragments will be amplified when the primer anneals on each strand over a length range . if sequence variation is present at the priming site , then a fragment may not be amplied , so the dna polymorphic can be detected Rapd (随机扩增多态性dna )技术是二十世纪90年代发展起来的一项dna分子多态性检测技术,它建立于聚合酶链式反应( pcr )技术基础之上,利用随机合成的寡聚核苷酸序列为引物(一般为10个bp ) ,分别与dna的两条单链结合,在dna聚合酶的作用下,对基因组的特定区域进行pcr扩增,其电泳结果为不同大小和数目的dna谱带即rapd图谱,可反映基因组相应区域的dna多态性。
And the genome dna of seven strains of spirulina platensis as mentioned above are analyzed with 106 random primers using random amplified polymorphic dna ( rapd ) technique . with the 106 random primers , 23 primers revealed their obvious polymorphisms and generated a total of 151 fragments , 120 of which were polymorphic 在此基础上,采用优化后的提取方法制备sp - d等7株钝顶螺旋藻的基因组dna ,利用106条随机引物对其进行rapd扩增,从中筛选出23条多态性引物,其扩增出的总条带数为151 ,多态性条带数为120 。
90 individuals of 6 populations , including possible wild populations and cultivated populations , with some individuals from inside and outside of china were analyzed by inter - simple sequence repeats ( issr ) and random amplified polymorphic dna ( rapd ) markers to determine the genetic diversity and genetic variations of among and within the populations . in addition , the status of ginkgo resource in china has been investigated in this study . 1 本研究对中国的银杏资源状况进行了调查,并采用issr和rapd两种分子标记技术,选取中国的6个银杏群体(包括可能的野生群体和栽培群体)以及国内外一些零星分布的银杏个体,从保护生物学角度对其遗传多样性水平和群体遗传结构进行了研究,获得以下结果。
Restriction fragment lenth polymorphism ( rflp ) analysis n random amplified polymorphic dna analysis [ rapd ] , the sequence analysis of internal transcribed spacers ( its ) of ribosomal dna ( rdna ) , induction of microcyclic conidiation , sem ( scanning electron microscopy ) ascosporal isolation and other methods were applied to study more than 100 specimens or isolates of cordyceps , its anamorphs and other entomogenous fungi 本文采用了rapd 、 rdnaits的rflp 、 rdna的its序列分析、诱发微循环产孢、扫描电镜观察、子囊孢子分离等方法研究了虫草及其他虫生真菌的100多个标本或菌株。
Species resources on chinese mitten - handed crab ( eriocheir sinensis ) have been intimately attached attention to in aquaculture . at first , morphological research was used , and isoenzyme and rapd ( random amplified polymorphic dna ) technique subsequently . these studies showed differences between different water systems from macrocosmic to microcosmic aspect 中华绒螯蟹种质资源的研究一直受到水产界人士的密切关注,从最初形态学的研究开始,经历了同功酶分析、 rapd技术分析等,这些研究从宏观到微观等不同的方面揭示了不同地理水系中华绒螯蟹之间的差异。
In this work , some species of palmae cultivated in the xiamen botanical garden had been selected to be analyzed their genetic diversity with rapd ( random amplified polymorphic dna ) technique . according to the result of genomic dna amplified with pcr ( polymerase chain reaction ) , genetic distance and similarity between different palm species were calculated on nei " s estimate of similarity and genetic distance . a primary but first time research at the phylogenetic relationships of some genera and species , the molecular classification and identification of some puzzling species of palmae was carried out through upgma ( unweighted pair group mean average ) cluster analysis of the genetic distance together with comparative study of the morphological structure characteristics 本文在初步调查分析了棕榈科植物在我国的自然分布、引种驯化情况以及该类植物在厦门地区栽培应用状况的基础上,首次采用rapd分子标记技术,对厦门万石植物园引种的一些棕榈科植物的遗传多样性进行了研究,根据pcr对基因组dna扩增的结果,用nei ' s相似性系数计算了不同植物间的遗传距离和遗传一致度,通过对遗传距离的upgma聚类分析,并结合形态分类的特点,对棕榈科植物的属、种间的系统分类关系和一些疑难种的分类鉴定进行了初步研究。