挑三拣四 pick and choose; be very particular [fastidious] in [about] (one's food, etc.) 不要挑三拣四的, 给什么就拿什么吧。 don't pick and choose, but take what's given to you
Through three rounds of screening , seventeen clones were selected and used in competitive test . the mab ge3 could specifically inhibit eight out of seventeen clones from binding to swine antisera . based on the amin acid sequences deduced from the foreign sequence inserted in the phage , it was indicated that all clones shared the core sequence - p / ekphf , that was similar to aa50 ~ aa55 domain of n protein of prrsv 从第三轮亲和筛选的噬菌体中随机挑取17个克隆进行功能鉴定,结果表明8个克隆与mabge3具有较强的特异性结合力并可以被prrsv阳性血清阻断,测序发现7个克隆具有核心序列: p ekphf ,该序列与prrsvn蛋白aa50 aa55 ( p ekphf )具有较高的同源性。
Based on cdna sequence of porcine defensin p - defensin - 1 gene reported in this study , we designed two pairs of primers with restriction enzyme sites , p - defensin - 1 gene was amplified by rt - pcr . the gene was inserted into expression vector and recombinant vector transformed into e . coli , which recombinant vector effectively produce fusion protein . finally , we hope antimicrobial peptide would be obtained from the fusion protein and explore the influence of the sequence of signal peptide on expression of antimicrobial peptide . methodrthe p - defensin - 1 gene which was amplified by rt - pcr ligated with vector pinpoint ? a - 3 which was proceedingly digested with restriction enzymes 方法:根据已报道的猪防御素- defensin - 1基因的cdna序列,设计二对带双酶切位点的引物,用rna提取试剂tripure从猪血白细胞中抽提rna ,通过rt - pcr方法扩增- defensin - 1基因,基因与载体pinpoint ~ ( tm ) xa - 3经酶切,连结,构建成重组质粒ppd - 1 、 ppd - 2 ,转化于大肠杆菌jm109中,经抽提质粒,酶切初步鉴定后,挑取阳性克隆,完成序列分析。
A cdna subtractive library with high subtractive efficiency of repeated + gz exposures in rat brain was constructed with suppression subtractive hybridization ( ssh ) . the cdna subtractive library after amplification included 100 blue clones and 400 white clones , 75 ones of which were selected to prepare for plasmid . identification of the clones with restriction endonuclease cleavage showed most of them had been cloned to the vector 构建高消减效率的+ gz重复暴露大鼠脑cdna消减文库,扩增后cdna文库包含约400个白色克隆和100个蓝色克隆,克隆饱满清晰,随机挑取75个白色克隆,制备质粒后,以ecor酶切分析,表明大部分克隆入质粒载体。
A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge 用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接;用连接产物转化大肠杆菌jml09感受态细胞,置含amp 、 x - gal和iptg的lb平板上培养12 20小时;挑取白色菌落于选择性培养基扩大培养,碱裂解法小量提取质粒dna ,并进行酶切分析鉴定,结果获得整合有prvge基因的重组质粒pugedna ,并与其它prv分离株进行ge基因序列同源性分析。
The e2 gene was amplified by rt - pcr , then examined the fragment by electrophoresis . after purification and insertion into pucm - t vecter , the recombinant plasmid pbne2pi and pbne2pii were obtained . then they were transfected e . coli jm109 and screened positive clones by blue or white plaques . the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis 通过rt - pcr扩增e _ 2基因,电泳测定扩增片段的大小,经纯化后,连接于pucm - t载体,获得重组质粒pbne2p 、 pbne2p ,转化e ? colijm109 ,经蓝白斑筛选,挑取阳性克隆,提取质粒,直接电泳鉴定和酶切鉴定。
Correlation analysis was done using the % vol of matched spots from the two group , which is a useful function offered by the software . consequently , the correlation coefficients of the plc - 1 + / + group were 0 . 858 , 0 . 867 , 0 . 875 , yet the other were 0 . 842 , 0 . 846 , 0 . 863 . we cut out 7 bad - matched spots ( exist in one group and out of the other ) after analysis by auto matching and manual correction , three of which has been analyzed by pmf up to now . of the three analyzed , one has been idendified as vimentin definitely , while the other two can not be identified 经过软件分析和手工修正,挑取了7个在两组凝胶中不能匹配的蛋白质斑点(一组存在而另一组消失) ,目前已对其中3个进行了质谱鉴定,其中一个确切鉴定为vimentin ,而另外两个不能得到鉴定。 vimentin是一种第三类中间纤维,与c - fos 、 c - jun和creb具有较高的同源性,在细胞分裂时常常大量地重新组织,发生显著的磷酸化。