Methods : methods of examination of diarrheogenic escherichia coli ( gb / t4789 . 6 - 2003 ) and isolation and enumeration of e . sakazakii from dehydrate infant formul recommended by fda of usa were applied 方法:参照gb / t4789 . 6 - 2003致泻性大肠埃希氏菌检验和美国fda 《婴幼儿奶粉中坂崎肠杆菌检测方法》部分。
The minimum inhibitory concentrations ( mic ) of imipenem , panipenem and meropenem for 225 clinical isolates was determined by agar dilution method , in comparison to 13 other antimicrobial agents 结果,三种碳青霉烯类抗生素对肠杆菌科细菌具高度抗菌活性,对铜绿假单胞菌、不动杆菌属、粪肠球菌等亦具良好抗菌作用。
3 r hpf4 was expressed as inclusion body in e . coli , after washing with reagent i ( 50mm tris - cl ph 8 . 0 ; 100mmnacl ; 0 . 5mm edta ; 2m carbamide ; 0 . 2 % tritonx - 100 ; 0 . 2 % doc ) and reagent ii ( 50mol / l , ph8 . 0 tris 3超卢结合溶由酶法破碎人肠杆菌后,经分析rhpf4主要以包涵体的形式存在,包涵体经洗涤液iuommtrk mphs
A total of 89 salmonella strains were isolated from poultry from beijing , tianjin , shandong etc . the resistance to 12 antimicrobial drugs and 6 fluoroquinolones were detected and analyzed by the broth microdilution method 建立了针对肠杆菌科细菌的耐药性监测方法-微量肉汤稀释法,并用该方法监测和分析了89株禽源沙门氏菌对12种抗菌素和6种氟喹诺酮类药物的耐药性。
Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted , and the target proteins expressed by iptg induced in escherichia coli . as well as in eukaryocyte ( hepg2 and cos - 7 ) , then their antigenity were detected 结论:截短的乙型肝炎表面抗原分子的原核和真核表达’重组质粒成功被构建及分别在人肠杆菌efl得到诱导表达和存贞核细胞ifj表达,并检测剑其表达产物的抗原特性。
However , the sequence indicated no homology that of cryla ( b ) and cryla ( a ) . sds - page showed that el - 1 and el - 2 expressed full - length 130kda protein in e . coll however , the recombinant plasmid from el - 1 and el - 2 , was transformed respectively by electi operation into acrystalliferous mutant bti ips 78 / ll ( serotype h14 ) and resulted in bl - i and bl - 2 2 .将p了夕和cy时基因以及它们重组休插人大肠杆菌表达载休puhe一24 ,分别获得了3种重组质粒plz19 、 plzcyta和休21ga .将重组质粒转化到不同大肠杆菌受休菌xli , dhs仪,获得了5种含有不同基因型的转化子
A database search revealed that the putative sequence of the red gene shows 40 - 50 % identity with those of uroporphyrinogen iii methyltransferase ( encoded by coba gene ) from various kinds of bacteria . an over - expression of the coba gene in e . coli was reported to lead to an accumulation of trimethylated derivative of porphyrin termed trimethylpyrrocorphin and factor ii , which emit strong red fluorescence under uv 在ddbj中搜索到多种细菌来源的coba基因(编码uroporph仰nogenhmethyltransferase )与redsene有40 50的同源性,并据报道,其中一个来源于pmpboibaclerilllaslldelll切chit的coba的基因,转人大肠杆菌、酵母菌及动物细胞后能使表达载体在紫外线下发射红色荧光。
Identification of functional bacteria showed predominant ammonifiers were shewanella , variovorax , chryseobacterium , bacillus or aeromonas ; among 4 selected nitrogen fixers , one ( azorhizobium caulinodans ) belonged to . a - proteobacteria , the other three ( serratia marcescens , klebsiella pneumoniae and citrobacter freundii ) were enterobacteriace , which belongs to - proteobacteria ; 2 nitrate reducers were aeromonas sp . and citrobacter sp . , 对各功能菌群中的优势菌的鉴定表明,优势的氨化细菌为希瓦氏菌属,产碱菌属,黄杆菌属,芽孢杆菌属或气单胞菌属;分离到的4个优势固氮细菌菌株中,一株为基瘤固氮根瘤菌,属于-变形菌亚门,而另外3株都属于肠杆菌科,归于-变形菌亚门。
Respectively . laboratory tests on phosphorus release abilities of selected bacterial strains showed ipb could liberate more than 50 g / ml , while opb produced less than 2 g / ml water soluble inorganic phosphorus ( wsip ) every day , demonstrating the enzymes participating in solubilization of ca - p should be constitutive ferments and enzymes concerning with mineralization of lecithin might be inducible catalysts . low abundance and poor compositions of nitrogen utilizing bacteria suggested poor bacterial diversities and weak self - purification of the lake 对3株有代表性的无机磷分解菌和6株占优势的有机磷分解菌的鉴定显示, 3株无机磷分解菌与巨大芽孢杆菌、大地杆菌和janthinobacteriumlividum最相近,而6株有机磷分解菌分别为固氮螺菌,产气肠杆菌, blastobacteraggregatus ,表皮葡萄球菌,溶血不动杆菌和念珠菌。
Fusion protein expression system can overcome those problem , increased the yield of yield of recombinant protein in e . coli . this remarkable increase in protein yield was thought to be due to protection of the target protein from proteolysis , improved folding , and efficient mrna translation . fusion protein also make the detection and purification easy , is a good strategies for achieving high - level expression of genes in escherichia coli 小分子量异源蛋自在人肠杆菌的表达受mrna不稳定、翻译起始效率低、易被蛋自酶降解等因素的干扰,较难获得高效表达,通过与已高表达的蛋自融合表达可以克服以上问题,可以使大多数蛋白获得高效表达。