These two subspecies are away from more than about 1 200 kilometers each other . the genomic dna of microtus fortis calamorum and microtus fortis fortis were extracted and amplified by pcr according to the specific genomic dna sequence of microtus fortis reported previously ( accession number in genbank : af277394 ) 根据东方田鼠基因组dna序列片段( genbank登录号: af277394 ) ,用pcr技术扩增东方田鼠长江亚种( microtusfortiscalamorum )和宁夏指名亚种( microtusfortisfortis )基因组dna ,得到一670bp左右的特异扩增片段。
In order to provide genetics basis for the classification of the subspecies of microtus fortis in china , the chromosome , isozyme , protein , dna were used to study the genetic characteristics and phylogenetic relationship of four groups of close - bred microtus fortis from three regions - hunan , ningxia as well as heilongjiang 本研究从染色体、同工酶及蛋白、 dna三个方面研究了来自湖南、宁夏和黑龙江三地的四类东方田鼠的遗传特性和亲缘关系,旨在为进一步明确我国东方田鼠的亚种分类提供遗传学依据。