Protein kinase b is newly found as a serine / threonine protein kinase , it could have widely spread functions in cell cycle regulation . the activation of protein kinase b / akt is thought to be a critical step in the phosphoinositide 3 - kinase pathway which regulates cell growth and differentiation 蛋白激酶c ( proteinkinasec , pkc )和蛋白激酶b ( proteinkinaseb , pkb )同属于丝苏氨酸蛋白激酶,广泛参与细胞的多种生命过程,包括生长、分化、细胞周期调控、细胞凋亡等。
It possess high homology with those of ck2 ( casein kinase 2 ) a subunits from various species . it is now well established that protein kinase ck2 is a pleiotropic and abiquitous serine or threonine kinase , which is highly conserved during evolution . we obtained the complete cdna sequence of ck2 a via 5 " race 酪蛋白激酶ck2是目前发现的普遍存在于真核生物中的丝氨酸苏氨酸型蛋白激酶,进化上具有高度的保守性并参与了多种生理功能的调控,因而克隆它的基因具有重要意义。
A combined metabolic mutated strain ( ys - c ) was get by traditional filter method from corynebacterium glutamicum ( ys ) . this mutant is threonine auxotrop , aec and ahv resistant , and it can grow in the medium which contants sca as the only source of carbon , and the mutant can endure hight leveal of leu . the mutanted strain can concentrate lysine . the concentration of lysine leveal was 1 . 20g / l 运用传统育种手段筛选到谷氨酸棒杆ys的多重代谢突变组合菌株ys - c 。该菌株为苏氨酸营养缺陷型,具有对赖氨酸的结构类似物aec和ahv的抗性,并且能以琥珀酸为唯一碳源生长,能够耐受高浓度亮氨酸。
Introduction protein kinase c ( pkc ) is a number of serine / threonine kinase family which play central regulatory roles in a multitude of cellular processes , including cell proliferation , cell cycle progression , differentiation , apoptosis . increasing evidence from studies using in vitro and in vivo systems suggests that pkc is a key regulator of critical cell cycle transitions , including cell cycle entry and exit and the gl and g2 checkpoint 前言细胞周期是细胞增殖、分化的一个重要过程,它的启动与进程受到严格调控,参与调控的因子不仅包含起正调控作用的细胞周期素( cyclins ) 、细胞周期蛋白依赖性激酶( cyclin - dependentkinase , cdk ) 、还有起负调控作用的cdk抑制因子( cyclin - dependentkinaseinhibitor , cki ) 。