Mechanism of glutamate - induced inhibition of pulmonary surfactant lipid synthesis ? mda receptor ( nmdar ) antagonist mk - 801 could reverse glutamate - induced inhibition of [ 3h ] - choline incorporation in lung explants ; ? he expression of nmdar1 subtype existed in alveolar type ii epithelial cells ; ? he effect of glu on pc synthesis required l - arginine in cultured lung explants , nos activity and no production were increased significantly in lung explants with glu treatment ; and mk - 801 and nos inhibitor l - nna could block the increase of nos activity and no production induced by glu ; l - nna also could reverse the inhibition of [ 3h ] - choline incorporation induced by glu ; ( 4 ) mda content increased and sod level decreased in cultured lung explants of glu treament groups , mk - 801 and l - nna could reverse these effects ; ( 3 ) mk - 801 , l - nna could block the release of ldh induced by glu 谷氨酸对肺表面活性物质脂质合成抑制的机制研究nmda受体拮抗剂mk - 801能逆转glu所致肺组织[ ~ 3h ] -胆碱掺入减少的效应;肺泡上皮细胞有nmda受体nmdar1亚单位的表达; glu对ps脂质合成的影响依赖l -精氨酸的存在, glu可使肺组织匀浆中nos活性、 no合成量升高; mk - 801 、 nos的抑制剂l - nna均可阻断glu所致的这些效应; l - nna可逆转glu所致肺组织[ ~ 3h ] -胆碱掺入减少的效应; glu可使肺组织匀浆中mda硕士学位论文中文摘要含量增高、 sod水平下降。 mk七0 、 l nna能阻断q所致的这些效应; mk80 、 l nna还能阻断gill诱导的ldh释放增多的效应。
3 . calcitonin gene - related peptide attenuates glutamate - induced inhibition of pulmonary surfactant lipid synthesis ( d cgrp could reverse the decrease of [ 3h ] - choline incorporation induced by glu with dose - dependence ; ( 2 ) cgrp could block the decrease of the content of cct a mrna induced by glu in lung tissue ; ( 3 } cgrp could reduce the impairment of the ultrastructure of at ii cells induced by glu ; ( 4 ) cgrp could reverse the increase of mda content and decrease of sod level induced by glu in cultured lung explants , respectively ; ? grp had no significant effect on nos activity and increase of no production induced by glu 降钙素基因相关肽减轻谷氨酸所致肺表面活性物质脂质合成抑制的保护作用降钙素基因相关肽grp )可显著减轻0所致肺组织h一胆碱掺入pc量的降低,并且呈剂量依赖保护效应; cgrp可逆转gill所致cctqinrna含量的降低; cgrp可减轻o所致肺11上皮细胞超微结构的损伤; cgrp可逆转o所致肺组织匀浆中mda含量增多、 sod水平降低的效应,并可逆转q所致肺组织ldh释放增多的效应; cg贩对gill引起的nos活性和no含量的升高均没有显著性影响。
Methods : in cultured lung explants without serum , the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity , nitric oxide ( no ) content , superoxide dismutase ( sod ) level , malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods . results : 1 . influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine , glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry , glu induced the changes of the ultrastruture of at ii iv cells 方法:采用成年大鼠肺组织无血清培养,运用[ ~ 3h ] -胆碱掺入法测定ps主要脂质磷脂酰胆碱( pc )合成量; rt - pcr扩增检测肺组织中pc合成限速酶磷酸胆碱二胞苷酰基转移酶( cct ) mrna含量;透射电子显微镜法观察肺泡型上皮细胞和ps系统超微结构的变化;免疫组织化学染色检测glu的受体nmdar1亚单位的表达;生化测定肺组织乳酸脱氢酶( ldh )释放量和肺组织匀浆中一氧化氮合酶( nos )活性、一氧化氮( no )生成量、超氧化物歧化酶( sod )水平以及丙二醛( mda )含量。
Abstract : this is a review with 27 references on the chemical constituents ( volatile and non - volatile components ) monoterpenes , sesquiterpenes , phenylpropanes , flavonoid , quinonoids , alkaloids , cholines and amino acids , etc . of rhizoma acori , including rhizoma acori graminei and rhizoma acori calami 文摘:菖蒲为较常用中药,具有多种药理及生理作用.含有单萜、倍半萜、苯丙素等挥发油成分及黄酮、醌、生物碱、胆碱、有机酸、氨基酸、糖等非挥发性成分.本文就此对两种菖蒲石菖蒲和水菖蒲的化学成分研究状况作一综述.以对其进行全面的研究与开发
Bsp ( boyine seminal plasma ) protein is the cardinal protein in bovine sperm , which can combine with choline or phosphycholine and then adhere to the surface of sperm to promote the sperm capacitation induced by heparin and high density lipoproteins ( hdl ) . moreover , bsp protein can combine with heterogeneous collagen , fibrinogen , heparin , calmoduline , igf - ii and apoa - i . bsp can also inhibit the migration of cholesterol on the sperm membrane 前言bsp ( bovineseminalplasma )蛋白是牛精浆中的主要蛋白质, bsp蛋白可以与胆碱或磷脂胆碱结合,黏附于精子表面,可促进由肝素、 hdl等诱导的获能过程,另外bsp蛋白与不同来源的胶原、纤维蛋白原、肝素、钙调蛋白、 igf - 、 apoa -等结合, bsp蛋白还可抑制精子胞膜上的胆固醇迁移,这些都暗示了bsp蛋白在精子胞膜的脂类代谢及调节精子的获能以及顶体反应方面发挥重要作用。
2 . ne - evoked contraction was not significantly changed after the extracellular na + was replaced by choline and c4h12n + , but was relaxed when the extracellular cl - was replaced by glutamate and gluconate . moreover , the vasoconstriction induced by ne was further enhanced with the replacement of the extracellular cl " by br " and scn " , which was still sensitive to either nfa or nppb 用氯化胆碱和四甲基氯化按分别替代灌流液中的氯化钠后血管张力无明显变化,在此基础上nfa 、 nppb仍能抑制血管收缩,引起血管完全舒张;用通透性低的阴离子谷氨酸根和葡萄糖酸根分别替代灌流液中的q后血管发生舒张;用通透性高的阴离子br和scn分别替代灌流液中的ci后血管张力增加,并能被nfa 、 nppb所抑制。
In higher plants , betaine is synthesized by a two - step oxidation of choline : choline - betaine aldehyde - betaine . the first step is catalyzed by choline monooxygenase ( cmo ) . the second step is catalyzed by betaine aldehyde dehydrogenase ( badh ) 高等植物中,甜菜碱由胆碱经两步酶催氧化得到,即:胆碱- -甜菜碱醛- -甜菜碱,催化第一步反应的酶是胆碱单加氧酶( cholinemonooxygenase , cmo ) ,催化第二步反应的酶是甜菜碱醛脱氢酶( betainealdehydedehydrogenase , badh ) 。
It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0 . 75 % . creatine , sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer , but creatinine and urea could not induce the creatinase production . 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation 最佳氮源为玉米浆和蛋白胨,最佳比例为2 : 3 ,最佳浓度为1 . 6 ;加入其它碳源时有助于菌株稳定产酶; 100ml摇瓶的最佳装液量为15ml ;肌酸、肌氨酸和氯化胆碱都能诱导菌株产酶,其中肌酸诱导产酶的效果最好,而肌酐和尿素不能诱导菌株产酶;诱导物肌酸的最适加入时间为接种培养12小时后,最适加入量为0 . 75 。
A mechanism that interprets this phenomenon was proposed as follows : the adsorbents can interact with phospholipid of ldl through calcium ion cross - linking of the phospholipid phosphate groups of ldl and the carboxyl groups of sde ligands . on the other hand , the electrostatic interaction between the positive charge of the phospholipid choline nitrogen of ldl and the negative charge of carboxyl groups of sde ligands is enhanced by the calcium cross - linking of the phosphate groups of neighboring phospholipids in ldl 其可能的原因是:在吸附体系中如存在大量的cay ,一方面ca可以作为桥梁连接ldl所含磷脂极性头部的磷酸基团和吸附微珠配体上的孩基;另一方面又可交联相邻磷脂间的磷酸基团,从而促进ldl所含磷脂极性头部的胆碱n ”与配体竣基的相互作用。
The fish lil liquid hard capsule selects the pure fish oil and the import lecithin which the non - pollution deep sea fish refinement becomes is the primary data , including the rich unsaturated fatty acid ( dha + epa ) and phosphor - lipin acid radical choline ( pc ) , is the young people and the child ce - rebrum grows the important nutrition ingredient 鱼油液体硬胶囊选用无污深海鱼提炼而成的精纯鱼和进口卵磷脂为主要原料,含丰富的不饱和脂肪酸( dha + epa )和磷脂酰胆碱( pc ) ,是青少年及儿童大脑成长中重要的营养成分。