Mitotic extracts were prepared from the synchronous metaphase plasmodia of physarum polycephalum . the results of western blotting indicated that cyclin b was a component of the mitotic extracts of physarum polycephalum 我们自同步化生长的多头绒泡菌中获得了有丝分裂中期提取物, westernblotting分析表明提取物中含有cyclinb 。
However , significant fraction of 3t3 - l1 preadipocytes differentiated into adipocytes without the mitotic clonal expansion when the preadipocytes were induced with the combination of 1 - isobutyl - 3 - methylxanthine and dexamethasone 用地塞米松和ibmx对细胞进行诱导不发生克隆扩增,而细胞也能部分分化,分化形成的脂肪细胞未经过dna复制。
Cultured epithelial cell undergoing division . this cell is in prophase of mitosis . microtubules are shown in green , actin is in red and mitotic chromosomes are colored blue 以上两图为正处于有丝分裂前期的动物上皮细胞。绿色的是微丝,红色为肌动蛋白,染色质高度螺旋成为粗短的染色体(蓝色) ,核膜核仁逐渐解体,染色体不规则地分布于细胞内。
Growth factors can act on many kinds of cells as mitotic promoter and deprival of growth factors can cause cease of cell growth or cell death . thus growth factors are usually thought relative to cell growth and differentiation 生长因子可以作为促分裂剂作用于多种细胞,剥夺生长因子可以导致细胞生长停止或细胞死亡,因此通常认为生长因子与促进细胞的增殖和存活有关。
Neurons are high1y differentiated ce11s in vivo , owing specific morpho1ogica1 structure and functions , and belonging to post - - mitotic ce11s , means that neurons once formed in the deve1oping course then can " t divided 为了探讨睡眠的功能,人们用剥夺睡眠的方法对人和动物进行了大量的研究。神经细胞是体内高度分化的有丝分裂后细胞,在发生过程中,一旦成熟它就失去了分裂能力。
Furthermore , when normally induced 3t3 - l1 preadipocytes were treated with pd98059 ( an inhibitor of mek - 1 ) , to block the activation of erk1 and 2 , the mitotic clonal expansion was blocked , while adipocyte differentiation was not affected 我们用有丝分裂原激活蛋白激酶激酶( mapkk / mek1 )的抑制剂pd98059与诱导分化激素及药物一起对细胞诱导分化,克隆扩增不发生,但细胞分化未受影响。
When the body suffered from malignancy , on the one hand , tumor restrained cell immunity function , antibody ' s production and the body ' s immunity effect through inducing restraining t cell , so it accelerated tumor ' s growth , on the other hand , tumor induced body fluid restrained gene , which prevented t cell and nk cell from killing tumor and restrained lymphocyte to mitotic multiplication reaction , indeed it accelerated tumor ' s growth 当机体一旦患上恶性肿瘤后,一方面肿瘤通过诱导抑制性t细胞,抑制细胞免疫功能及抗体的产生,抑制机体对肿瘤的免疫效应,从而使肿瘤逃逸机体的免疫监视,促进肿瘤的生长。另一方面,肿瘤通过诱导体液抑制因子阻止t细胞和nk细胞的等对肿瘤的杀伤作用,抑制淋巴细胞对有丝分裂原的增殖反应,甚至促进肿瘤的生长。
In the laboratory experiment part , human peripheral blood , cultured cells and icr mice were study objects . the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test . the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test . the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry , western blot and rt - pcr 流行病学结果显示长期小剂量辐射接触与染色体不分离呈正相关,为进一步在细胞遗传学和分子生物学方面研究小剂量电离辐射与染色体不分离关系及其机制,本课题第二部分以外周血、培养细胞、 icr小鼠为研究对象,用外周血染色体计数和单细胞受精卵染色体计数的方法研究小剂量辐射和拓扑异构酶复旦大学2000级博士生学位论文11a抑制剂及其二者的协同效应对有丝分裂和减数分裂染色体不分离的影响,用免疫细胞化学染色、 westernblot 、 rt pcr等方法研究了电离辐射引起拓扑异构酶a表达变化。
After the nuclei of late g2 phase were incubated in the mitotic extracts harvested from metaphase plasmodia , the cyclin b in the nuclei gradually accumulated with the prolongation of incubating time . while , in the control group , the content of cyclin b changed little . this indicated that the mitotic extracts of metaphase plasmodia had the activity of accelerating the process of prophase , and the cell free system with mpf activity was established 将g _ 2晚期细胞核温育于中期提取物中,免疫印迹结果表明随着温育时间的延长,细胞核中cyclinb的含量逐渐增加,而在没有中期提取物的对照组细胞核中cyclinb含量并不随着温育时间的延长而增加,说明多头绒泡菌中期提取物具有促进有丝分裂前期进程的作用,同时也说明我们构建了具有mpf活性的非细胞体系。
The nuclei of the late g2 phase treated by cytochalasinb were incubated in the mitotic extracts harvested from the metaphase plasmodia . western blotting analysis showed that the content of cyclin b in the nuclei changed little . in contrast , in the control group , the content of cyclin b gradually increased with the prolongation of incubating time 将经细胞松弛素b处理后的g _ 2晚期细胞核在有丝分裂中期提取物中温育,经洗涤后对细胞核中cycli b的含量进行分析,发现随着温育时间的延长,细胞核中cyclinb的含量没有明显的变化。