sequence n. 1.继续;接续;连续。 2.顺序;程序;次第;关系;关联。 3.后果;结果;接着发生的事;后事;后文。 4.【数学】数列;序列;数贯。 5.【无、计】指令序列;定序。 6.【计算机】顺序机〔将信息项目排成顺序的机器〕。 7.【音乐】用不同音调反复演奏一组乐句。 8.【天主教】宣讲福音前唱的圣歌。 9.【牌戏】顺。 10.【电影】(描述同一主题的)连续镜头;片断,插曲;场景。 a logical sequence 条理;逻辑顺序。 a causal [physical] sequence 因果关系。 the natural sequence to [for] folly 愚笨行为的必然结果。 in rapid sequence 一个接着一个;紧接着。 in regular sequence 挨次;按次序;逐一;有条不紊地。 in sequence 挨次;顺次;逐一。 sequence of tenses 【语法】时态的配合[接续,呼应]。
2 . firstly , the vector pcambia2301g with two gus reporter genes was constructed . then one gus was substituted with the coding sequence of dsg 10 obtained by pcr , and pcambia2301g - dsg10 was constructed . finally the pcambla2301g - dsg10 was introduced into agrobacterium tumefaciens using the freeze - thaw method 3 2 、首先构建了中间载pcambia2301g ,再用它和dsg10构建了可在植物中高效表达dsg10的载体pcambia2301g - dsg10 ,并将该质粒导入根癌农杆菌( agrobacteriumtumefaciens ) eha105中。
Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3 . the recombinant plasmid was transformed to e . coli bl21 and the gst / mpem fusion protein was induced to express with iptg . the fusion protein was purified by affinity chromatography 方法: pcr扩增小鼠pemcdna编码序列,将它克隆到含有gst的原核表达质粒载体pgex - 4t - 3上,转化大肠杆菌bl21 ,诱导表达gst mpem融合蛋白,通过亲和层析,获得初步纯化的产物,以羟胺切割验证其一级结构。
Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing 目的构建蛇毒锯鳞蝰素( echistatin )的原核高效表达体系方法由genebank数据库检索蛇毒锯鳞蝰素( echistatin )的氨基酸序列,结合大肠杆菌蛋白质合成体系对氨基酸密码子使用的偏爱性,设计了echistatin编码基因,体外人工合成编码基因dna片段,通过适当的限制性内切酶位点插入表达载体pbv220 ,分别构建了echistatin的单拷贝表达克隆、双拷贝串联表达克隆;进一步通过pcr技术构建echistatin的融合表达基因克隆。
Rlean meat percentage is one of the most important economic traints in pig breeding programs . myostatin is a negative regulator of skeletal muscle growth . null or low activity of myostatin , individual muscle of mutant amimals would show a large and widespread increase in skeletal mass . myostatin null animals have significantly larger diameter or more quantity of fiber skeletal muscle . the phenotype was termed double muscling . in order to probe the relation between myostatin and high lean meat rate and plump - hipped trait , we sythesized the c ' 80 amino acids coding sequence of porcine myostatin and costructed the cloning and expressing vector of it 肌生成抑制素( myostatin ,即mstn )是近几年来( mcpherrona . c等, 1997 )发现的骨骼肌生长的负调控因子,它主要在骨骼肌中表达。其活性的丧失,会引起动物肌肉的过度发育,肌纤维直径变大或肌纤维数增加,表现为双肌症状。肌生成抑制素研究的突破将对猪、肉鸡、肉牛等畜禽生产性能的提高具有特别重要的意义。
In addition , the nt - 3 and nt - 4 coding sequences were used to resolve the taxa problems of the giant panda and the red pandacaizurus fulgens ) , combining with mtdna genetic marker and other traditional taxonomic data . the result suggested that the giant panda should be classified as a separate subfamily within ursidae , while the red panda should be included in a monotypic family ailurdae 4基因的保守性,结合线粒体dna上的序列和其它传统分类学资料,对大熊猫及小熊猫的分类地位进行了探讨,认为应将小熊猫单独列为一科,大熊猫与小熊猫关系较远,应划入熊科。
In the experiment , the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked . it was expressed in e . coli and its protein was determined . after having been properly modified , the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing , which is the prerequisite work for genetic transformation 本实验从抗除草剂转基因bobwhite小麦中,利用pcr克隆的方法扩增出bar基因全长,并在原核表达系统中表达,鉴定表达蛋白的活性,将能够正确编码ppt乙酰转移酶的bar基因片段,经过适当的修饰构建入真核表达载体。