Mix the pcr products and the hybridization buffer, cover each microarray, preserve in the capsule, 55 c, 30 min to hybridize pcr产物加杂交液稀释混匀,覆盖于各微阵列,置人杂交舱中55保温3omin。
At the technology of microarray, increasing the hybridizing signal will enhance the reliability of gene's expression of the ratio of variety 摘要在基因芯片技术中,提高杂交信号有利于增加基因表达变化比值的可信度。
Such notable professionals as john rivers hybridized various tree fruits, and introduced superior clones from france and elsewhere 著名的专业工作者如johnrivers对各种果树进行了杂交,并从法国和其他地方引进优良的无性系。
Ffuorescence was observed in the cytoplasm and the region near the nucleolus of the cell . ms showed that mb hybridized wth the expression of ingl 传感器对cdna的响应时间为15分钟,并对单碱基突变的dnaz具有很高的选择性。
7 . the labeled sample was purified and hybridized with the self-made cdna microarray, which demonstrated low background and clear positive signals 7、标记的样品纯化后与芯片杂交,获得的杂交结果背景低,阳性信号的界限清楚。
By researching how spotting affect the hybridizing signal after dissolving pcr products with different impregnant, we hope to optimize the technology of microarray 通过研究不同溶剂来溶解pcr产物后,点样对杂交信号的影响,使基因芯片的技术环节得到优化。
Not only limited in the hybridizing of the conventional girder and arch, the hybrid system arch bridges nowadays have had new hybridizing of arches with other types of structures 组合体系拱桥已不仅限于传统的梁拱组合,还出现了拱与各种结构形式的新型组合。
Not only limited in the hybridizing of the conventional girder and arch, the hybrid system arch bridges nowadays have had new hybridizing of arches with other types of structures 组合体系拱桥已不仅限于传统的梁拱组合,还出现了拱与各种结构形式的新型组合。
Then we study the methods of image analysis and data analysis in order to extract the hybridizing intensity and retrieve the biological information 然后从图像分析和数据分析两方面研究了影响芯片分析的因素,实现了对杂交荧光图像的信号提取和基本的生物信息分析。
Tester cdnas were divided into two groups and ligated to the specific adaptor 1 and adaptor 2r, and then hybridized with normal aloe vera l cdna twice with two rounds of suppression pcr 同样用northernblotting对几种胁迫条件下,库拉索芦荟nadp一苹果酸酶基因a叻贸的表达情况进行了分析。