Experiments of nucleic acid hybridization show that taqman 6 only hybridizes to the pcr products amplified from s . costatum, but not to products from other microalga . these results indicate that primer6 ( f / r ) and taqman6 can be used to establish the rfq-pcr method for the quantitative detection of s . costatum 最后以primer6rdna序列在几种浮游植物的分类及中肋骨条藻定量检测中的应用(f/r)和taqman6探针建立了定量检测中肋骨条藻的rfq一pcr。
It was suggested that eric-pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively; 3 in this study, genetic diversity in a . polytricha was higher than that in a . auricula : 4 in this study, a . fuscosuccinea had a higher homology to a . auricula than to a . polytricha; 5 morphological characteristics validated the results from eric-pcr and provided a potential explanation for the higher similarity coefficient between a . auricular and a . fuscosuccinea; 6 southern hybridization was employed by choosing a strain from a . auricula as a probe which hybridized with a . auricula and a . fuscosuccinea except a . polytricha, further confirming the veracity of the results from eric-pcr; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity 本研究中，木耳属2个种的2个菌株在its区域表现出较高的保守性，4种限制型内切酶的酶切图谱没有显示出多态性；增加内切酶种类及供试菌株数量，有可能获得具有多态性的限制性内切酶酶切图谱；9本实验中，its区域的真菌特异性引物与真核生物通用引物对于扩增效果无较大差异，扩增片段长度均为650bp左右；10根据形态学实验、eric-pcr实验以及southern杂交实验的结果分析，紫木木耳属种质资源的遗传鉴定和遗传多样性评价耳极有可能是毛木耳种的一个变种;n.本研究中所用的gutc法是一种适用于木耳属菌株基因组洲a快速提取的方法;12.传统的形态学分类法和现代的分子生物学分类法，两者的关系是相辅相成，互为验证
Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102-iii biosynthesis . so the 300kb fragment was recovered and used as probe to hybridize with 10-22 genomic library . cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids 以此片段为探针钓出了位于该区域的文库克隆，并利用指纹印迹和杂交技术将这些文库克隆排列起来，进而以位于该区域的不同位置的片段做臂构建了用于转化和接合转移用的基因置换质粒，并试图通过基因置换将该区域置换下来，但尚未得到最终结果。
Finally, genetic optimization research is summarized on several typical production scheduling problems . after expounding the general idea of genetic algorithm, the comparative advantages in contrast to the traditional algorithm, the basic characteristics of genetic algorithm and its theoretical base, the paper puts emphasis on the efficiency of genetic algorithm in the scheduling of flow shop, and puts forward an improving genetic algorithm : the ordinal genetic algorithm based on the heuristic rules . the new algorithm introduces into the initial group the solution of heuristic algorithm, and in the group structure adopts a strategy of first ordering according to the priority of the adaptive solution, and then defining a new way of choosing probability by segments, which provides more hybridizing opportunity for optimized individuals, and designs variation-control rule to prevent single population and partial optimal solution 在论述了遗传算法的思想、与传统搜索算法的比较优势、遗传算法的基本特征和遗传算法的理论基础(包括模式定理、隐含并行性、基因块假设、欺骗问题和收敛性定理)后，重点探讨了遗传算法在flowshop调度问题中的潜力和有效性；结合启发式规则，提出了一个改进的遗传算法?基于启发式规则的有序遗传算法，新算法在初始种群中引入了启发式算法的解，在种群结构上采用了先按适应值优劣排序再分段确定选择概率的新策略，使优质个体有更多的杂交机会，在变异中设计了变异控制规则，以防种群单一化，而陷入局部优化解。
The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1, respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus, which had no analogical to human gene . mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell 第三章，根据一种常见的病毒烟草花叶病毒(tmv)的核酸序列设计了分子信标荧光探针，由于tmv的遗传物质是rna，分子信标又具有很高的特异性和灵敏度，因此感染了病毒粒子的植物叶片在经过简单处理后，可用分子信标检测叶片上。
Abstract : according to the characteristic of hybridize and sudden change of binary coding chromosome in classical genetic algorithm, this paper defines the rule of hybridize and sudden change of continuous variables . the method is noted for easy to operate and quite good to approach the best true solution in overall situation . by means of three computed examples of nonlinear optimization and quadratic programme problem, it confirms that the method has strong adaptability and can precisely determine the best solution, in overall situation 文摘:根据传统遗传算法中对于二进制编码染色体的杂交和突变的特点，定义了连续变量的杂交、突变规则.此方法有操作简便，可较好地逼近全局最优的真实解的特点.通过非线性优化和二次规划问题的3个算例，证实本算法适应性强，可较精确地确定全局最优解
Abstract : according to the characteristic of hybridize and sudden change of binary coding chromosome in classical genetic algorithm, this paper defines the rule of hybridize and sudden change of continuous variables . the method is noted for easy to operate and quite good to approach the best true solution in overall situation . by means of three computed examples of nonlinear optimization and quadratic programme problem, it confirms that the method has strong adaptability and can precisely determine the best solution, in overall situation 文摘:根据传统遗传算法中对于二进制编码染色体的杂交和突变的特点，定义了连续变量的杂交、突变规则.此方法有操作简便，可较好地逼近全局最优的真实解的特点.通过非线性优化和二次规划问题的3个算例，证实本算法适应性强，可较精确地确定全局最优解
Ny13 fragment had positive signals hybridized with line 14 and leymus multicaulis, which proved that it was a responsive gene in salt stress . furthermore, we tested ny 13 with southern blotting, and also found positive signal in the leymus muhicaulis . this segment may be correlated with the addition chromosome and has the character of salt-resistance 继续回收杂交信号处的ona片段，连接到可转化人l_染色体(tac)载体上，构建成含有多枝赖草盐胁迫应答基因的tac克隆，以期今后通过耐盐功能互补实验验证而直接用于基因_程育种，井可进一步筛选分离获得耐盐相关基因。
According to marketing segmentation theory and positional theory, the paper considers the corporation ’ s ability and resource and chooses the professional hybridized pig culturist and scattered hybridized pig culturist . after making a market research and gaining the data and using the joint-analytical method of orthogonal design method to calculate the product preference of market culturist, then comparing the market position of competitor, the paper ensures kangda corporation ’ s product position to supply the differential marketing strategy 在此分析基础上,本文根据市场细分和定位理论,结合公司的能力和资源,评估和选择了土杂猪专业户和土杂猪散户这两个具有发展潜力的细分市场,并根据市场调查获得的数据,采用基于正交设计的联合分析法分别获得了目标市场养殖户购买偏好,结合竞争对手的市场定位,确定了公司市场定位,为后续的差异化营销策略制定提供支撑。
