ligase造句

• We have identified in s . cerevisiae distinct ubiquitin - ligase complexes that define different erad pathways
  我们在酵母体内发现了一种参与不同erad途径的特定的泛素连接酶复合体。
• Ligase an enzyme that catalyzes the bond formation between two substrates at the expense of the breakdown of atp or some other nucleotide triphosphate
  连接酶：可将两个底物结合在一起的酶，此过程需要atp或其他核苷三磷酸供能。
• Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants
  将cdna与载体连接，并导入dh5感受态细胞中，构建成cdna文库。
• As bases are added by polymerase to the starting point of a new complementary strand , known as a primer , or recognized by ligase as a match , the template ' s sequence is revealed
  当聚合酶将一个核苷酸加在新互补链的起始引子之后，或接合酶认定某段核苷酸链与原始模版配对，就可利用这些反应来得出原始模版的序列。
• 4 . the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi , and collected the digested cpti fragment and the pgem - 7z , then ligated by t4 dna ligase and formed the pgem - cp
  中间载体及表达载体的构建将puc - cp质粒和pgem ? 7z质粒，用kpni和bamhi酶切，分别回收cpti片断和酶切后的载体片段，用t _ 4连接酶连接构建成中间载体pgem - cp 。
• Sub - - clone of s , . / hbsag fusion gene : pbuescripts , . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme , and were linked under t4 dna ligase , ppiczaa s , , / hbsag was constructed and transformed to e . coli
  Hbsag质粒与ppiczaa载体分别经xhol和xbaln切，再在t4dna连接酶作用下进行连接，获得工程菌表达型ppiczaas ； hbsag质粒，转化大肠杆菌t0p10细胞，经xhol和xbal与sacll和xbal酶切电泳，证实s ； 。
• Cut off beta fragment from plasmid prok . ii with hindlll and ecor i as insert , and cut pa into linear plasmid as vector fragment . link the insert and vector fragment together with t4 ligase , and the new vector with gene beta and gus was constructed
  用hind和ecor双酶切prok质粒，获得beta基因片段作为插入片段，用hind和ecor双酶切a质粒作为载体片段，将插入片段与载体片段相连，即构建成含有beta和gus的双基因载体。
• At first . then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr . following that , these gene fragments and plasmid vectors , pbluescript ii ks + and puc18 , were cut by bamh i and kpn i . the prepared insert dna and vector dna were linked by t4 dna ligase
  利用vectornti6 . 0软件，对所克隆的序列用相邻接点法( neighborjoining州j ） method ）进行多序列比对，分析其同源性，并构建基因进化树。
• The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme , were linked by means of t4 dna ligase and transformed into e . coli jm109 permissive cells , yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
  将重组质粒pugedna与转移载体pfastbacldna用bamhi和ecori双酶切处理， t _ 4dna连接酶连接，用连接产物转化大肠杆菌jm109感受态细胞，得到重组转移载体质粒pfastbac - gedna 。
• This study demonstrated that the arabidopsis f - box protein coil associated with atcul1 , atrbxl and skpl - like proteins askl and ask2 to assemble scfcoil ubiquitin ligase complexes . also , we found that the atcull component of scfcoil complexes contained two species including atcull and modified atcull . ( 2 ) we found that coil assembled to two separate scfcoil complexes with either askl or ask2 through immunoprecipitation analysis with plant expressing myc - tagged version of ask2
  用表达融合蛋白myc - ask2的拟南芥为材料，以- myc抗体进行免疫共沉淀分析发现， myc - ask2蛋白可以与coi1蛋白一起免疫共沉淀，但是不能与ask1蛋白免疫共沉淀，表明coi1蛋白与ask2蛋白，但是不能同时与ask1结合形成scf ~ ( coi1 )复合体。
• After digested with ecori and noti , the obtained dna fragment was linked with ppic9k by t4dna ligase and then the recombinant plasmid was transformed into competent dhscc . after positive transformants were sieved out by pcr , digesiting analysis and sequencing were also used to confirm the positive result more
  所得的dna片段经ecor和not双酶切后用t _ 4dna连接酶与ppic9k载体进行连接，然后导入大肠杆菌dh5 ，用pcr法筛选阳性转化子，并用双酶切和序列测定方法鉴定重组质粒。
• This time , using cdna of zmcdc5 as template , we amplify a sequence by means of pcr technology . and then , using restrict endoenzyme and ligase , we conjunct the 0 . 8kb length dna sequence in a expression vector , pet - 30a . after induction , expression and purification , we obtained a 35 . 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ) . with the purified protein , we got its antibody and testified the antibody by means of western blotting and dot blotting
  本实验是以zmcdc5的cdna为模板，使用pcr获得基因片段，再通过酶切连接，将得到的0 . 8kb的基因片段构建于pet - 30a表达载体上，经过诱导表达和纯化，获得zmcdc5的融合蛋白，其中包括了zmcdc5925个氨基酸中647 914共267个氨基酸残基
• A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge
  用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接；用连接产物转化大肠杆菌jml09感受态细胞，置含amp 、 x - gal和iptg的lb平板上培养12 20小时；挑取白色菌落于选择性培养基扩大培养，碱裂解法小量提取质粒dna ，并进行酶切分析鉴定，结果获得整合有prvge基因的重组质粒pugedna ，并与其它prv分离株进行ge基因序列同源性分析。
• The scfco , " ubiquitin - ligase complexes are required for jasmonate response in arabidopsis ( l ) arabidopsis coi is required for all the jasmonate - regulated response . it encodes a protein containing leucine - rich repeats and a degenerate f - box motif these structural features are characteristic of f - box proteins that function in ubiquitin ligase complexes for the ubiquitylation of substrate - proteins targeted for degradat1on
  以表达融合蛋白flag - coi1的拟南芥为材料，用- flag抗体进行免疫共沉淀分析发现， coi1蛋白可以与ask1 、 ask2 、 atrbx1和atcul1蛋白结合形成scf ~ ( coi1 )复合体，并发现其中的atcul1蛋白包括未修饰和修饰的两种形式。
• First , the purified pezzis and pcr product of angiostatin are digested by ecor . i and xba i . after purifying the digested products respectively , we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as . then transform it to the competent e . coli dh5a
  用限制性内切酶ecori与xbai对目的基因as 、表达载体pezz18行双酶切，酶切产物纯化后利用大肠杆菌t _ 4dna连接酶连接构成重组子pezz18 - as ，并转化e . colidh5 ，经氨苄青霉素lb平板初筛后，以菌液pcr和重组子的单、双酶切行进一步鉴定。