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核酸探针的英文

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"核酸探针"怎么读用"核酸探针"造句

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  • nucleic-acid probe

例句与用法

  • Nonradioactive nucleic probe to tgev was prepared and was applicated to detect tgev at first stage , which established foundational work for differentiating tgev from prcv and investigation on epidemiology of tgev
    制备了tgev非放射性标记的核酸探针,并初步应用于检测tgev 。二者为tgev鉴别诊断、血清学调查奠定了基础。
  • The specificity of the nucleic acid probe was very strict . lt reacted positively with iltv dna only and it react negatively with the nuleic acid of ndv , bv and ibdv . the sensitivity of this kind of probe is very high . ilt could even detect 20pg ' s iltv dna
    结果表明:该种核酸探针具有高度的特异性,它仅与iltv的dna呈现阳性反应,而与新城疫病毒、传染性法氏囊病病毒和传染性支气管炎病毒的核酸等均呈阴性反应。该种探针具有高度的敏感性,能够检测到20pg的iltv的dna 。
  • Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
    最后,用地高辛随机引物法标记成momp基因核酸探针,斑点杂交检测衣原体基因组dna ,灵敏度可达10pg ,且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较,初步证明该探针具有较高的敏感性与较强的特异性。
  • The author reviewed the detection measures of prunus necrotic ringspot virus , and related the research progression of the pathogen detection technology inside and outside . the template amplication technology include pcr assays ^ nasba and so on . the cdna and crna probe which labeled with the isotope % biotin or dig , the offset probe and the peptide probe can be applied to magnify the signal . pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification
    本文回顾了李坏死环斑病毒的检测方法,较全面地评述了国内外病原物检测技术研究进展:在模板的扩增有各种pcr技术、 nasba技术;在信号的放大有同位素、生物素或dig标记的cdna和crna探针,分支探针和肽核酸探针;模板扩增和信号放大相结合的有pcr -基因扫描技术、 pcr安捷伦芯片实验室技术;模板扩增和杂交以及信号放大相结合的有pcr - elisa技术、实时荧光pcr技术、生物芯片技术。
  • One was cloning , sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ) , the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot
    为了鉴别诊断tgev与prcv及对tgev进行流行病学调查,本研究采用原核表达系统( gst融合表达系统)表达tgev纤突蛋白( s蛋白)中含有b和c抗原位点的多肽,并且制备了非放射性地高辛标记的核酸探针,通过斑点杂交( dot - blot )检测tgev核酸rna 。
  • Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 %
    本研究中首次对iltv - nm98a株的tk基因进行了克隆和序列分析,结果表明: iltv - nm98a株tk基因的核苷酸序列与已发表的iltvtk基因的核苷酸序列具有高度的同源性,两者之间仅相差4个核苷酸,同源性高达99 . 7 ,从而证实了iltvtk基因是高度保守的,为iltvtk基因核酸探针的制备提供了有力的依据。
  • In this study , the meaningful results have been achieved , which is the important basic work to research the pili subunit vaccine of avian e . coli , and detect pila gene by nucleric acid probe . moreover , it is significant to research molecular epidemiology , to diagnose , to prevent and treat avian colibacillosis
    本研究为鸡源致病性大肠杆菌菌毛基因工程疫苗的研究,制备核酸探针检测pila基因提供了重要材料,对鸡大肠杆菌病的分子流行病学研究、诊断和防治研究具有重要意义。
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