轻链可变区的英文

• variable region of light chain
• variable region of the light chain

• "轻"英文翻译    light
• "链"英文翻译    chain
• "可变"英文翻译    variable; changeable
• "区"英文翻译    H region; H
• "链可变区" 英文翻译 :    gly4ser
• "重链可变区" 英文翻译 :    variable region of heavy chain; variable region of the heavy chain
• "可变区" 英文翻译 :    region of variability; variable area; variable range; variable region
• "链可变菌素" 英文翻译 :    streptovarycin
• "单链可变片段" 英文翻译 :    single chain variable fragment
• "v-区,可变区" 英文翻译 :    v-region
• "高度可变区" 英文翻译 :    hypervariable region
• "可变区基因" 英文翻译 :    v-gene
• "可变区取样" 英文翻译 :    variable plot sampling
• "可变区群" 英文翻译 :    variable region group
• "可变区亚群" 英文翻译 :    variable region subgroup
• "可变区域" 英文翻译 :    variable area
• "可变区域；变面积" 英文翻译 :    va
• "可变区域轨迹" 英文翻译 :    variable-area track
• "可变区域单元问题" 英文翻译 :    modifiable areal unit problem
• "轻链" 英文翻译 :    l chain; l-chain; light chain
• "单链抗体可变区基因片段" 英文翻译 :    single-chain variable fragments
• "超变区" 英文翻译 :    hypervariable region
• "多变区" 英文翻译 :    diversity region
• "高变区" 英文翻译 :    highly variable region; hypervariable region
• "渐变区" 英文翻译 :    transition region

• The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
  取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。
• Thus , immunologists have sought smaller molecules with the antigen - recognition capability of antibodies . the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable . it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
  二、日本血吸虫单克隆杭独特型抗体np30的单链狐（ scf ）的构建、表达及对balbic小鼠诱导保护性作用研究l 、日本血吸虫单克隆杭独特型抗体np0的单链抗体归cfv ）的构建、表达通过pcr方法体外扩增并经测序验证的重链、轻链可变区（ vh 、 vl ）基因先后重组入原核表达质粒ptha90相应的位点上，中间通过一连接肽（ gly在er ） 。
• In the present study , the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis . total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies . after obtained using rpas system , vh and vl genes were used to assemble scfv gene fragment with a linker primer
  应用重组噬菌体抗体库技术，从分泌小鼠抗牛精子sp18抗体的杂交瘤细胞系中分离总rna ，克隆抗体重链和轻链可变区基因，加入连接肽引物( linkerprimer )组装成单链抗体scfv ( singlechainfragmentvariable )基因并用rs引物进行扩增， sfi 、 not酶切，回收后与pcantab5e载体相连，转化e . colitg1宿主菌，构建单链抗体文库。
• Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
  Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ，反转录成cdna ；利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因，并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因；将mg _ 7单链抗体基因插入pcantab5e ；将连接产物转化感受态tg1大肠杆菌，制备细菌形式的mg _ 7重组噬菌体抗体库；通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。